Openspritzer

Full MS link: http://biorxiv.org/content/early/2016/12/13/093633
Open-Labware link: https://open-labware.net/projects/openspritzer/

Openspritzer: an open hardware pressure ejection system for reliably delivering picolitre volumes.

Chris J Forman, Hayley Tomes, Buchule Mbobo, Tom Baden, Joseph V Raimondo

The ability to reliably and precisely deliver picolitre volumes is an important component of biological research. Here we describe a high-performance, low-cost, open hardware pressure ejection system (Openspritzer), which can be constructed from off the shelf components. When connected to a standard micro-pipette via suitable pneumatic tubing, the device is capable of delivering minute doses of reagents to a wide range of biological and chemical systems. In this work, we characterise the performance of the device and compare it to a popular commercial system using two-photon fluorescence microscopy. We found that Openspritzer provides the same level of control over delivered r